pACEBac1-gamma-TuSC
(Plasmid #178079)

• Purpose: Insect cell expression of gamma-TuSC
• Depositing Lab: Tarun Kapoor
• Publication: Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 10.1083/jcb.202009146.

Backbone

• Vector backbone: pACEBac1
• Backbone manufacturer: Geneva Biotech
• Total vector size (bp): 10949
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: gamma-tubulin
• Species: H. sapiens (human)
• Insert Size (bp): 1353
• Entrez Gene: TUBG1 (a.k.a. CDCBM4, GCP-1, TUBG, TUBGCP1)
• Tag / Fusion Protein:
  • TEV-His6 (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: GCP2
• Species: H. sapiens (human)
• Insert Size (bp): 2790
• Entrez Gene: TUBGCP2 (a.k.a. ALP4, GCP-2, GCP2, Grip103, PAMDDFS, SPBC97, SPC97, Spc97p, h103p, hGCP2, hSpc97)

Gene/Insert 3

• Gene/Insert name: GCP3
• Species: H. sapiens (human)
• Insert Size (bp): 2721
• Entrez Gene: TUBGCP3 (a.k.a. 104p, ALP6, GCP3, Grip104, SPBC98, Spc98, Spc98p)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Assembled via BstXI/Iceu-I restriction digestion following the MultiBac manual