pET30-R5-GFP
(Plasmid #17838)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pET30
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5422
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Cloning in E.coli DH10B Protein expression in E.coli BLR(DE3)
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    pET30-GFP
  • Alt name
    pET30-R5-GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    779
  • GenBank ID
    AJ851288
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • S-tag (N terminal on backbone)
    • R5 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Shaikh, A. S., Tang, Y. J., Mukhopadhyay, A., Keasling, J. D. “Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein”. Anal Chem. 2008; 80(3):886-890

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-R5-GFP was a gift from Jay Keasling (Addgene plasmid # 17838)
  • For your References section:

    Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein. Shaikh AS, Tang YJ, Mukhopadhyay A, Keasling JD. Anal Chem. 2008 Feb 1. 80(3):886-90. 10.1021/ac071445+ PubMed 18179240