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Plasmid 17838: pET30-R5-GFP
  • pET30-GFP

  • pET30-R5-GFP

  • 779

  • Aequorea victoria

  • AJ851288

  • His

  • N terminal on backbone

  • S-tag

  • N terminal on backbone

  • R5

  • N terminal on insert

  • pET30
    (Search Vector Database)

  • Novagen

  • Bacterial Expression, SynBio

  • 5422

  • NcoI

  • No

  • EcoRI

  • No

  • T7 List of Sequencing Primers

  • Kanamycin

  • DH5alpha

  • 37

  • Cloning in E.coli DH10B Protein expression in E.coli BLR(DE3)

  • High Copy

  • View sequences (2)
  • View map

  • Jay Keasling

  • UBMTA
    EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Shaikh, A. S., Tang, Y. J., Mukhopadhyay, A., Keasling, J. D. “Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein”. Anal Chem. 2008; 80(3):886-890

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein. Shaikh et al (Anal Chem. 2008 Feb 1. 80(3):886-90. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17838" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only