GFP-GGA1
(Plasmid #178459)

• Purpose: Expresses GGA1 in mammalian cells
• Depositing Lab: Juan Bonifacino
• Publication: Puertollano et al Mol Biol Cell. 2003 Apr;14(4):1545-57. doi: 10.1091/mbc.02-07-0109.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6621
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GGA1
• Species: H. sapiens (human)
• Insert Size (bp): 1920
• Mutation: See Depositor Comments Below
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: GFP-For
• 3′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a S434L mutation in GGA1 compared to the NCBI reference sequence NP_037497.1. This mutation is not known to affect plasmid function.