pJH1969
(Plasmid
#178900)
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PurposePnmr-1::D3cpv unc-54 3' UTR C.elegans neural expression of Pnmr-1D3cpv
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178900 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBSK
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Backbone manufacturerstratagene
- Backbone size w/o insert (bp) 6731
- Total vector size (bp) 8690
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameD3cpv
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Insert Size (bp)1959
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GenBank ID
- Promoter Pnmr-1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CGTCGACGgatcccatggtgagcaagggcg
- 3′ sequencing primer CAGTTGGAATTcttactcgatgttgtggcgg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH1969 was a gift from Mei Zhen (Addgene plasmid # 178900 ; http://n2t.net/addgene:178900 ; RRID:Addgene_178900) -
For your References section:
An imbalancing act: gap junctions reduce the backward motor circuit activity to bias C. elegans for forward locomotion. Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M. Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005. 10.1016/j.neuron.2011.09.005 PubMed 22099460
Map uploaded by the depositor.
