pTSB-FICAT_Payload-Esp3I
(Plasmid
#179380)
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Purpose(Empty Backbone) Transposon vector prepared for Esp3I golden gate cloning of Transgene
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179380 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePB512
- Backbone size (bp) 4972
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Modifications to backboneRemoved mcs, EF1a RFP to include Esp3I restriction sites for cloning of any desired gene for targeted delivery
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Golden gate piggyback transposon backbone to clone transgene for targeted transgene insertion.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTSB-FICAT_Payload-Esp3I was a gift from Marc Güell (Addgene plasmid # 179380 ; http://n2t.net/addgene:179380 ; RRID:Addgene_179380) -
For your References section:
Find and cut-and-transfer (FiCAT) mammalian genome engineering. Pallares-Masmitja M, Ivancic D, Mir-Pedrol J, Jaraba-Wallace J, Tagliani T, Oliva B, Rahmeh A, Sanchez-Mejias A, Guell M. Nat Commun. 2021 Dec 3;12(1):7071. doi: 10.1038/s41467-021-27183-x. 10.1038/s41467-021-27183-x PubMed 34862378
Map uploaded by the depositor.
