pET-epELM-5
(Plasmid #179391)

• Purpose: Express a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteria
• Depositing Lab: Shiro Iuchi
• Publication: Iuchi et al Commun Biol. 2021 Dec 15;4(1):1399. doi: 10.1038/s42003-021-02928-y.

Backbone

• Vector backbone: pET28C (+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5367
• Total vector size (bp): 5504
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB C3013
• Growth instructions: Depositor recommends NEB C3013 for downstream applications.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ELMSAN1
• Alt name: MIDEAS
• Species: H. sapiens (human)
• Insert Size (bp): 153
• GenBank ID: NM_194278.3
• Promoter: T7 lac
• Tag / Fusion Protein:
  • 6x His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: T7 promoter primer
• 3′ sequencing primer: T7 terminator primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert encodes the epitope (ELMSAN1 residue 550-600) of the antibody (Catalog # A303-157A, BETHYL). Expressed protein has been confirmed by Western blot.