Addgene - pME1mL1 Plasmid Data

Plasmid 17971: pME1mL1

Gene/insert name:
pmyc1tetO-lacZ, tetR(B)
Alt name:
LacZ
Vector backbone:
pMS2
(Search Vector Database)
Vector type:
mycobacteria expression
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Hygromycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
hygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria, This plasmid is high copy number in E. coli and low copy number in mycobacteria.
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Sabine Ehrt
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

Expresses lacZ under control of tetR(B). Also see Pubmed ID 15687379.

Derivative of pMS2 with lacZ under the control of Pmyc1tetO and tetR under the control of Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Silencing Mycobacterium smegmatis by using tetracycline repressors. Guo et al (J Bacteriol. 2007 Jul . 189(13):4614-23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17971" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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