Plasmid 17971: pME1mL1
  • pmyc1tetO-lacZ, tetR(B)

  • LacZ

  • pMS2
    (Search Vector Database)

  • mycobacteria expression

  • n/a List of Sequencing Primers

  • Hygromycin

  • DH5alpha

  • 37

  • hygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria, This plasmid is high copy number in E. coli and low copy number in mycobacteria.

  • High Copy

  • View sequences (3)
  • View map

  • Sabine Ehrt



Expresses lacZ under control of tetR(B). Also see Pubmed ID 15687379.

Derivative of pMS2 with lacZ under the control of Pmyc1tetO and tetR under the control of Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Silencing Mycobacterium smegmatis by using tetracycline repressors. Guo et al (J Bacteriol. 2007 Jul . 189(13):4614-23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17971" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only