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Plasmid 17971: pME1mL1
  • pmyc1tetO-lacZ, tetR(B)

  • LacZ

  • pMS2
    (Search Vector Database)

  • mycobacteria expression

  • n/a List of Sequencing Primers

  • Hygromycin

  • DH5alpha

  • 37

  • hygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria, This plasmid is high copy number in E. coli and low copy number in mycobacteria.

  • High Copy

  • View sequences (3)
  • View map

  • Sabine Ehrt

  • UBMTA

Comments: 

Expresses lacZ under control of tetR(B). Also see Pubmed ID 15687379.

Derivative of pMS2 with lacZ under the control of Pmyc1tetO and tetR under the control of Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Silencing Mycobacterium smegmatis by using tetracycline repressors. Guo et al (J Bacteriol. 2007 Jul . 189(13):4614-23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17971" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only