|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17971||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typemycobacteria expression
Growth in Bacteria
Growth instructionshygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria, This plasmid is high copy number in E. coli and low copy number in mycobacteria.
Copy numberHigh Copy
Gene/Insert namepmyc1tetO-lacZ, tetR(B)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Expresses lacZ under control of tetR(B). Also see Pubmed ID 15687379.
Derivative of pMS2 with lacZ under the control of Pmyc1tetO and tetR under the control of Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pME1mL1 was a gift from Sabine Ehrt (Addgene plasmid # 17971)
For your References section:Silencing Mycobacterium smegmatis by using tetracycline repressors. Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein M, Ehrt S, Schnappinger D. J Bacteriol. 2007 Jul . 189(13):4614-23. 10.1128/JB.00216-07 PubMed 17483222