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(Plasmid #17971)


Item Catalog # Description Quantity Price (USD)
Plasmid 17971 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    hygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria, This plasmid is high copy number in E. coli and low copy number in mycobacteria.
  • Copy number
    High Copy


  • Gene/Insert name
    pmyc1tetO-lacZ, tetR(B)
  • Alt name

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Expresses lacZ under control of tetR(B). Also see Pubmed ID 15687379.

Derivative of pMS2 with lacZ under the control of Pmyc1tetO and tetR under the control of Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME1mL1 was a gift from Sabine Ehrt (Addgene plasmid # 17971)
  • For your References section:

    Silencing Mycobacterium smegmatis by using tetracycline repressors. Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein M, Ehrt S, Schnappinger D. J Bacteriol. 2007 Jul . 189(13):4614-23. 10.1128/JB.00216-07 PubMed 17483222