Addgene - GFP-Bcl2 Plasmid Data

Plasmid 17999: GFP-Bcl2

Gene/insert name:
Bcl-2
Alt name:
Bcl2
Insert size:
720
Species:
H. sapiens (human)
Entrez Gene:
BCL2 (Bcl-2, PPP1R50)
Fusion protein or tag:
GFP
Terminal:
N terminal on backbone
Vector backbone:
pEGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
Bgl II
Site destroyed during cloning:
No
Cloning site 3':
Eco RI
Site destroyed during cloning:
No
5' sequencing primer:
CATGGTCCTGCTGGAGTTCGT List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
human Bcl-2 was PCR amplified from pB4 plasmid purchased from ATCC
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Clark Distelhorst
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Transient expression of wild-type or mitochondrially targeted Bcl-2 induces apoptosis, whereas transient expression of endoplasmic reticulum-targeted Bcl-2 is protective against Bax-induced cell death. Wang et al (J Biol Chem. 2001 Nov 23. 276(47):44117-28. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17999" in your Materials and Methods section.