Addgene - pWZL Hygro- TRF2 Plasmid Data

Plasmid 18009: pWZL Hygro- TRF2

Gene/insert name:
hTRF2
Alt name:
human TTAGGG repeat binding factor 2
Alt name:
2455
Insert size:
1497
Species:
H. sapiens (human)
Entrez Gene:
TERF2 (TRBF2, TRF2)
Mutation:
aa2-500
Vector backbone:
pWZL Hygro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5900
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
CCTTTGTACACCCTAAGCCT List of Sequencing Primers
3' sequencing primer:
AATGCTCGTCAAGAAGAC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Titia de Lange
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Senescence induced by altered telomere state, not telomere loss. Karlseder et al (Science. 2002 Mar 29. 295(5564):2446-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18009" in your Materials and Methods section.