pcDNA-MYC-As-NF-kB1
(Plasmid #180203)

• Purpose: Expresses MYC-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells
• Depositing Lab: Thomas Gilmore
• Publication: Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi: 10.1038/s42003-021-02924-2.

Backbone

• Vector backbone: pcDNA-MYC
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6750
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: As-NF-kB1
• Alt name: Acanthoeca spectabilis NF-kB1
• Species: Acanthoeca spectabilis
• Insert Size (bp): 1224
• Mutation: aa 2-409
• Promoter: CMV
• Tag / Fusion Protein:
  • MYC-tagged fusion protein (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GenScript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Codon optimized for human cell expression