Addgene - Phleo/Xpress Plasmid Data

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1805: Phleo/Xpress
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Plasmid 1805: Phleo/Xpress

Gene/insert name:
Phleo/Xpress
Insert size:
922
Fusion protein or tag:
Xpress
Terminal:
C terminal on insert
Vector backbone:
pBluescript II KS
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Bacterial Expression
Backbone size w/o insert:
3000
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
Phleomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Christian Tschudi
Terms and Licenses:
MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: In vivo epitope tagging of Trypanosoma brucei genes using a one step PCR-based strategy. Shen et al (Mol Biochem Parasitol 2001 Mar;113(1):171-3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1805" in your Materials and Methods section.