Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

BLA/V5
(Plasmid #1806)

Add to Cart
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBluescript II KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    BLA/V5
  • Insert Size (bp)
    1009
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BLA/V5 was a gift from Christian Tschudi (Addgene plasmid # 1806)
  • For your References section:

    In vivo epitope tagging of Trypanosoma brucei genes using a one step PCR-based strategy. Shen S, Arhin GK, Ullu E, Tschudi C. Mol Biochem Parasitol 2001 Mar;113(1):171-3. 10.1016/S0166-6851(00)00383-2 PubMed 11254965