Addgene - Lamp1-CFP Plasmid Data

Plasmid 1815: Lamp1-CFP

Gene/insert name:
lysosome associated membrane protein 1
Alt name:
Lamp-1
Insert size:
1200
Species:
R. norvegicus (rat)
Entrez Gene:
Lamp1 (LGP120)
Fusion protein or tag:
CFP
Terminal:
C terminal on backbone
Vector backbone:
pECFP-N1
(Search Vector Database)
Backbone manufacturer:
BD Biosciences / Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-N List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Norma Andrews provided Lamp-1
Sequence: View sequences (3)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Walther Mothes
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1815" in your Materials and Methods section.