Friend MLV Env YFP
(Plasmid #1820)

• Depositing Lab: Walther Mothes
• Publication: Sherer et al Traffic 2003 Nov;4(11):785-801.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Envelope
• Alt name: Env
• Alt name: FrMLVgp3
• Species: MuLV
• Insert Size (bp): 2800
• Entrez Gene: env (a.k.a. FrMLVgp3)
• Tag / Fusion Protein:
  • EYFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ? (unknown if destroyed)
• 3′ cloning site: ? (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Abraham Pinter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

YFP was cloned into the NheI site of pFr-MuLV273/274 from Abraham Pinter. The entire Env gene was then transferred into pcDNA3.