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Addgene

XRE-H2B-eGFP
(Plasmid #182294)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182294 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    H2B-GFP
  • Backbone manufacturer
    Geoff Wahl Lab (Addgene #11680)
  • Backbone size w/o insert (bp) 1646
  • Total vector size (bp) 6157
  • Modifications to backbone
    CMV promotor was removed and replaced by XRE sequence.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xenobiotic Response Element
  • Alt name
    XRE
  • Alt name
    CYP1A1 promotor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1646
  • Entrez Gene
    CYP1A1 (a.k.a. AHH, AHRR, CP11, CYP1, CYPIA1, P1-450, P450-C, P450DX)
  • Promoter XRE
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AseI (unknown if destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer ATTAATGCTTCTGGCACATAGTAGTT
  • 3′ sequencing primer AGATCTGAGCTCAGCTGGGAAG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XRE-H2B-eGFP was a gift from Severine Degrelle & Thierry Fournier (Addgene plasmid # 182294 ; http://n2t.net/addgene:182294 ; RRID:Addgene_182294)
  • For your References section:

    Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR). Degrelle SA, Ferecatu I, Fournier T. Environ Int. 2022 Sep 24;169:107545. doi: 10.1016/j.envint.2022.107545. 10.1016/j.envint.2022.107545 PubMed 36179647