pSEM371 - ce-unc-119 array integration
(Plasmid
#182640)
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Purpose(Empty Backbone) Co-injection fragment for extra-chromosomal array integration at ce-unc-119 locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182640 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTwist-Amp-high-copy
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Backbone manufacturerTwist Biosciences
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Unpublished reagents. Sonia el Mouridi and Christian Froekjaer-Jensen. Synthetic Genome Biology lab. KAUST.
www.wormbuilder.org
MOdular Safe harbor Transgene Insertion (mosTI).
Transgene fragment for array insertion into ce-unc-119 locus. Use with pSEM376 (sgRNA spacer: TTTGGGAACCAGGTGTTGG)
Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEM371 - ce-unc-119 array integration was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 182640 ; http://n2t.net/addgene:182640 ; RRID:Addgene_182640) -
For your References section:
Modular safe-harbor transgene insertion (MosTI) for targeted single-copy and extrachromosomal array integration in C. elegans. El Mouridi S, Alkhaldi F, Frokjaer-Jensen C. G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 10.1093/g3journal/jkac184. 10.1093/g3journal/jkac184 PubMed 35900171