pBY011-AMN1ko
(Plasmid #183099)

• Purpose: S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.
• Depositing Lab: Gabor Balazsi
• Publication: Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi: 10.1038/s42003-022-03228-9.

Backbone

• Vector backbone: pBY011
• Backbone manufacturer: Harvard Medical School
• Backbone size w/o insert (bp): 3607
• Total vector size (bp): 5972
• Modifications to backbone: None
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AMN1 (left homology arm) - KanMX - AMN1 (right homology arm)
• Alt name: AMN1 (YBR158W)
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2365
• Mutation: only includes external sequences (504 bp each) as homology arms, separated by KanMX
• GenBank ID: EEU05423 EEU05423.1
• Entrez Gene: AMN1 (a.k.a. YBR158W, CST13, ICS4)
• Promoter: URA3, TEF1
• Tag / Fusion Protein:
  • KanMX

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAACTAACAAAAACGTTCAAAAAGTTT
• 3′ sequencing primer: AACACTATGAGTGTATCTTTTGGAG

Resource Information

• Supplemental Documents:
  • pBY011-AMN1ko.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry