pBTK150
(Plasmid
#183126)
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PurposeBTK Type 2 promoter part for golden gate assembly
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183126 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonecolE1
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameReverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression
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SpeciesSynthetic
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Insert Size (bp)141
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBTK150 was a gift from Jeffrey Barrick (Addgene plasmid # 183126 ; http://n2t.net/addgene:183126 ; RRID:Addgene_183126) -
For your References section:
Engineered symbionts activate honey bee immunity and limit pathogens. Leonard SP, Powell JE, Perutka J, Geng P, Heckmann LC, Horak RD, Davies BW, Ellington AD, Barrick JE, Moran NA. Science. 2020 Jan 31;367(6477):573-576. doi: 10.1126/science.aax9039. Epub 2020 Jan 30. 10.1126/science.aax9039 PubMed 32001655