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Addgene

pBTK150
(Plasmid #183126)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 183126 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    colE1
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression
  • Species
    Synthetic
  • Insert Size (bp)
    141

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTK150 was a gift from Jeffrey Barrick (Addgene plasmid # 183126 ; http://n2t.net/addgene:183126 ; RRID:Addgene_183126)
  • For your References section:

    Engineered symbionts activate honey bee immunity and limit pathogens. Leonard SP, Powell JE, Perutka J, Geng P, Heckmann LC, Horak RD, Davies BW, Ellington AD, Barrick JE, Moran NA. Science. 2020 Jan 31;367(6477):573-576. doi: 10.1126/science.aax9039. Epub 2020 Jan 30. 10.1126/science.aax9039 PubMed 32001655