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pRET.IL.PGK-TK
(Plasmid #1837)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pRET
  • Vector type
    Mammalian Expression, Retroviral, Cre/Lox
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    retroviral RET construct
  • Alt name
    removable exon trap
  • Alt name
    Type I vector

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

See scanned map (#5).
This is a modified RET vector exclusively for the DEL.BANK project (JC Schimenti, the Jackson Lab): the PGK promoter for the HSV-TK gene is much stronger than the MC1 promoter in other RET vectors. Virus production is virtually impossible. Either the PGK promoter or PGK poly A signal seems to be very inhibitory for the transcription of the virus genome. You can easily excise the essential components as an XbaI-XbaI fragment.
(Leder #C-1024)

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRET.IL.PGK-TK was a gift from Philip Leder (Addgene plasmid # 1837)
  • For your References section:

    RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Ishida Y, Leder P. Nucleic Acids Res 1999 Dec 15;27(24):e35. 10.1093/nar/27.24.e35 PubMed 10572187