1
Plasmid 1837: pRET.IL.PGK-TK
  • None

  • retroviral RET construct

  • removable exon trap

  • Type I vector

  • pRET
    (Search Vector Database)

  • Mammalian Expression, Retroviral, Cre/Lox

  • na List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Neomycin

  • View sequences (1)
  • View map

  • Philip Leder

  • UBMTA

Comments: 

See scanned map (#5).
This is a modified RET vector exclusively for the DEL.BANK project (JC Schimenti, the Jackson Lab): the PGK promoter for the HSV-TK gene is much stronger than the MC1 promoter in other RET vectors. Virus production is virtually impossible. Either the PGK promoter or PGK poly A signal seems to be very inhibitory for the transcription of the virus genome. You can easily excise the essential components as an XbaI-XbaI fragment.
(Leder #C-1024)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Ishida et al (Nucleic Acids Res 1999 Dec 15;27(24):e35. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1837" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only