Addgene - pRET.IL.PGK-TK Plasmid Data

Plasmid 1837: pRET.IL.PGK-TK

Gene/insert name:
None
Alt name:
retroviral RET construct
Alt name:
removable exon trap
Alt name:
Type I vector
Vector backbone:
pRET
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral, Cre/Lox
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Philip Leder
Terms and Licenses:
MTA

Comments:

See scanned map (#5).
This is a modified RET vector exclusively for the DEL.BANK project (JC Schimenti, the Jackson Lab): the PGK promoter for the HSV-TK gene is much stronger than the MC1 promoter in other RET vectors. Virus production is virtually impossible. Either the PGK promoter or PGK poly A signal seems to be very inhibitory for the transcription of the virus genome. You can easily excise the essential components as an XbaI-XbaI fragment.
(Leder #C-1024)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Ishida et al (Nucleic Acids Res 1999 Dec 15;27(24):e35. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1837" in your Materials and Methods section.