pHAGE-EGFP-SKL
(Plasmid
#183727)
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PurposeFor exrpression of GFP targeted to peroxisomes
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Depositing Lab
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Depositing OrganizationWeizmann Institute of Science
Located in Israel -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183727 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepHAGE
- Backbone size w/o insert (bp) 7835
- Total vector size (bp) 8564
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
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Insert Size (bp)717
- Promoter UbiC
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Tag
/ Fusion Protein
- SKL (peroxisomal targeting sequence 1) (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer -
- 3′ sequencing primer -
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.06.622258 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE-EGFP-SKL was a gift from Jeffrey Gerst (Addgene plasmid # 183727 ; http://n2t.net/addgene:183727 ; RRID:Addgene_183727) -
For your References section:
Complementation of a human disease phenotype by intercellular mRNA transfer. Haimovich G, Dasgupta S, Govindan Ravi A, Gerst JE. Cell Rep. 2026 Sep 7;45(9):117959. doi: 10.1016/j.celrep.2026.117959. 10.1016/j.celrep.2026.117959 PubMed 42704713
