pEFBOS_CreIRESpuro
(Plasmid
#183812)
-
PurposeExpresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepBR322/pUC
- Total vector size (bp) 8097
-
Vector typeMammalian Expression, Synthetic Biology
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCre recombinase
-
Alt nameCre
-
SpeciesBacteriophage P1
-
Insert Size (bp)1029
-
Tag
/ Fusion Protein
- NLS
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the polyA region has ambiguous bases in the Addgene NGS result due to difficulties sequencing through this region (4940-5005 bp).
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEFBOS_CreIRESpuro was a gift from Ed Stanley (Addgene plasmid # 183812 ; http://n2t.net/addgene:183812 ; RRID:Addgene_183812) -
For your References section:
A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis. Davis RP, Costa M, Grandela C, Holland AM, Hatzistavrou T, Micallef SJ, Li X, Goulburn AL, Azzola L, Elefanty AG, Stanley EG. Nat Protoc. 2008;3(10):1550-8. doi: 10.1038/nprot.2008.146. 10.1038/nprot.2008.146 PubMed 18802436
