pLenti-EML4-ALK variant 3a I1171N
(Plasmid #183832)

• Purpose: Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells
• Depositing Lab: Aaron Hata
• Publication: Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s43018-022-00399-6. doi: 10.1038/s43018-022-00399-6.

Backbone

• Vector backbone: pLenti
• Backbone size w/o insert (bp): 7877
• Total vector size (bp): 10235
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ALK Receptor Tyrosine Kinase
• Alt name: ALK
• Species: H. sapiens (human)
• Insert Size (bp): 1691
• Mutation: I1171N
• GenBank ID: NM_004304
• Entrez Gene: ALK (a.k.a. ALK1, CD246, NBLST3)
• Promoter: CMV
• Tag / Fusion Protein:
  • The fusion protein of EML4 exon 1-6a and ALK exon 20-29

Cloning Information for Gene/Insert 1

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: EMAP Like 4
• Alt name: EML4
• Species: H. sapiens (human)
• Insert Size (bp): 667
• GenBank ID: NM_019063.5
• Entrez Gene: EML4 (a.k.a. C2orf2, ELP120, EMAP-4, EMAPL4, ROPP120)
• Promoter: CMV
• Tag / Fusion Protein:
  • The fusion protein of EML4 exon 1-6a and ALK exon 20-29

Cloning Information for Gene/Insert 2

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry