Akaby
(Bacterial strain #184224)

• Purpose: Akaby is a RecB knockout E. coli strain, made for cell-free expression system for linear templates.
• Depositing Lab: Kate Adamala
• Publication: Sato et al PLoS One. 2022 Apr 7;17(4):e0266272. doi: 10.1371/journal.pone.0266272. eCollection 2022.

Backbone

• Vector backbone: none

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Akaby (NEB 5-alpha derivative)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ∆RecB (RecB gene was replaced with a kanamycin resistant gene, KanR)

Resource Information

• Supplemental Documents:
  • Akaby Colony PCR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Colony PCR Primers:
(1) ATTTCTTCCATCAGGCGGT
(2) CAGTCATAGCCGAATAGCCT
(3) CGGTGCCCTGAATGAACTGC
(4) GTCCCTCTCCGGCATCATGA
Expected product size: 661bp with primer (1) and (2), 1035bp with primer (3) and (4).

Please visit https://www.biorxiv.org/content/10.1101/2021.11.03.467179v1 for bioRxiv preprint.