BMP10 N67Q N131Q D4G (S2-2)
(Plasmid #184622)

• Purpose: for transfection into S2 cells and expression of BMP10 protein
• Depositing Lab: Timothy Springer
• Publication: Le et al J Mol Biol. 2022 Mar 15;434(5):167439. doi: 10.1016/j.jmb.2021.167439. Epub 2022 Jan 4.

Backbone

• Vector backbone: pExpreS2-2
• Backbone size w/o insert (bp): 2799
• Vector type: Insect Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BMP10
• Species: H. sapiens (human)
• Insert Size (bp): 1475
• Mutation: N67Q, N131Q, ARIRR(312-316) replaced with G
• Entrez Gene: BMP10
• Tag / Fusion Protein:
  • 8x His - streptavidin binding peptide (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba1 (unknown if destroyed)
• 3′ cloning site: Not1 (unknown if destroyed)
• 5′ sequencing primer: CGT CTA CGG AGC GAC AAT TC
• 3′ sequencing primer: CAC GCG CTT GAA AGG AGT GT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry