pTriEx4_Rap1
(Plasmid
#184662)
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PurposeMammalian expression of mouse Rap1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTriEx4
- Backbone size w/o insert (bp) 5159
- Total vector size (bp) 6359
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namerap1
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SpeciesM. musculus (mouse)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CTGTAAATTACATTTTATTTACAATCAAAGGAG
- 3′ sequencing primer CCTGAGGTTAATCACTTAGTGATG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.11.02.467017v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTriEx4_Rap1 was a gift from Agnel Sfeir (Addgene plasmid # 184662 ; http://n2t.net/addgene:184662 ; RRID:Addgene_184662) -
For your References section:
Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states. Barry RM, Sacco O, Mameri A, Stojaspal M, Kartsonis W, Shah P, De Ioannes P, Hofr C, Cote J, Sfeir A. Genes Dev. 2022 Feb 24. pii: gad.349039.121. doi: 10.1101/gad.349039.121. 10.1101/gad.349039.121 PubMed 35210222
Map uploaded by the depositor.
