pLVX-MPro-eGFP-2
(Plasmid #184824)

• Purpose: Expresses Mpro-eGFP in mammalian cells
• Depositing Lab: Wenshe Liu
• Publication: Cao et al ACS Cent Sci. 2022 Feb 23;8(2):192-204. doi: 10.1021/acscentsci.1c00910. Epub 2022 Feb 2.

Backbone

• Vector backbone: PLVX-puro
• Backbone size w/o insert (bp): 8102
• Total vector size (bp): 10493
• Modifications to backbone: added EF-1alfa promoter and IRES
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mpro-eGFP conjugate
• Alt name: Mpro-eGFP
• Species: SARS-CoV-2, jellyfish Aequorea Victoria
• Insert Size (bp): 1706
• Entrez Gene: M (a.k.a. GU280_gp05)
• Promoter: EF-1alfa

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CTAGTTCTAGAatgAAAACAAGCGC
• 3′ sequencing primer: GCGGGATCCGCGGC

Resource Information

• Supplemental Documents:
  • pLVX-EF1alpha-cut3CLproEGFP-IRES-Puro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry