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pLVX-MPro-eGFP-2
(Plasmid #184824)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 184824 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PLVX-puro
  • Backbone size w/o insert (bp) 8102
  • Total vector size (bp) 10493
  • Modifications to backbone
    added EF-1alfa promoter and IRES
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mpro-eGFP conjugate
  • Alt name
    Mpro-eGFP
  • Species
    SARS-CoV-2, jellyfish Aequorea Victoria
  • Insert Size (bp)
    1706
  • Entrez Gene
    M (a.k.a. GU280_gp05)
  • Promoter EF-1alfa

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CTAGTTCTAGAatgAAAACAAGCGC
  • 3′ sequencing primer GCGGGATCCGCGGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-MPro-eGFP-2 was a gift from Wenshe Liu (Addgene plasmid # 184824 ; http://n2t.net/addgene:184824 ; RRID:Addgene_184824)
  • For your References section:

    Evaluation of SARS-CoV-2 Main Protease Inhibitors Using a Novel Cell-Based Assay. Cao W, Cho CD, Geng ZZ, Shaabani N, Ma XR, Vatansever EC, Alugubelli YR, Ma Y, Chaki SP, Ellenburg WH, Yang KS, Qiao Y, Allen R, Neuman BW, Ji H, Xu S, Liu WR. ACS Cent Sci. 2022 Feb 23;8(2):192-204. doi: 10.1021/acscentsci.1c00910. Epub 2022 Feb 2. 10.1021/acscentsci.1c00910 PubMed 35229034