Talin Head
(Plasmid
#186140)
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PurposeFor expression of recombinant Talin head domain in bacteria
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186140 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-30b
- Backbone size w/o insert (bp) 5420
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTLN1
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SpeciesH. sapiens (human)
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Insert Size (bp)1368
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Entrez GeneTLN1 (a.k.a. ILWEQ, TLN, talin-1)
- Promoter Lac
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Tag
/ Fusion Protein
- His6 (tev cleavage) (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Talin Head was a gift from Michael Rosen (Addgene plasmid # 186140 ; http://n2t.net/addgene:186140 ; RRID:Addgene_186140) -
For your References section:
Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation. Case LB, De Pasquale M, Henry L, Rosen MK. Elife. 2022 Jan 20;11. pii: 72588. doi: 10.7554/eLife.72588. 10.7554/eLife.72588 PubMed 35049497
