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Purpose(Empty Backbone) KanR-BBR1 entry vector for HR donor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186238 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBBR1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cloning entry vector using BbsI, Empty BBR1 backbone with RFP dropout site
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBA707 was a gift from Jennifer Doudna (Addgene plasmid # 186238 ; http://n2t.net/addgene:186238 ; RRID:Addgene_186238) -
For your References section:
Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing. Adler BA, Hessler T, Cress BF, Lahiri A, Mutalik VK, Barrangou R, Banfield J, Doudna JA. Nat Microbiol. 2022 Dec;7(12):1967-1979. doi: 10.1038/s41564-022-01258-x. Epub 2022 Oct 31. 10.1038/s41564-022-01258-x PubMed 36316451