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Addgene

pDF0428 huDisCas7-11-R1045-GGGS-R1122-U6-pro-Gluc
(Plasmid #186985)

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Item Catalog # Description Quantity Price (USD)
Plasmid 186985 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    N/A
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cas7-11-r1045-gggs-r1122, Gluc crRNA guide
  • gRNA/shRNA sequence
    crRNA: gTTGatgtcacggaacTGCAGCCAGCTTTCCGGGCATTGGCTTCCATttttttt
  • Species
    D. ishitomonii
  • Mutation
    r1045-gggs-r1122 deletion

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains an A1290V mutation in cas7-11-r1045-gggs-r1122 and an 18bp deletion immediately downstream of the crRNA sequence. These differences are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDF0428 huDisCas7-11-R1045-GGGS-R1122-U6-pro-Gluc was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 186985 ; http://n2t.net/addgene:186985 ; RRID:Addgene_186985)

  • For your References section:

    Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex. Kato K, Zhou W, Okazaki S, Isayama Y, Nishizawa T, Gootenberg JS, Abudayyeh OO, Nishimasu H. Cell. 2022 May 25. pii: S0092-8674(22)00581-5. doi: 10.1016/j.cell.2022.05.003. 10.1016/j.cell.2022.05.003 PubMed 35643083
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