Addgene - Cdk5-HA Plasmid Data

Plasmid 1872: Cdk5-HA

Gene/insert name:
Cdk5
Alt name:
cyclin-dependent kinase 5
Alt name:
PSSALRE
Insert size:
910
Species:
H. sapiens (human)
Entrez Gene:
CDK5 (PSSALRE)
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Vector backbone:
pCMV-neo-Bam
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6550
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
Bglob-intron-F List of Sequencing Primers
3' sequencing primer:
Bglob-pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Sander van den Heuvel
Terms and Licenses:
MTA

Comments:

See scanned map.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Distinct roles for cyclin-dependent kinases in cell cycle control. van et al (Science 1993 Dec 24;262(5142):2050-4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1872" in your Materials and Methods section.