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Cdk4-HA
(Plasmid #1876)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1876 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-neo-Bam
  • Backbone size w/o insert (bp) 6550
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdk4
  • Alt name
    cyclin-dependent kinase 4
  • Alt name
    PSK-J3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    940
  • Entrez Gene
    CDK4 (a.k.a. CMM3, PSK-J3)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer Bglob-pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See scanned map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cdk4-HA was a gift from Sander van den Heuvel (Addgene plasmid # 1876 ; http://n2t.net/addgene:1876 ; RRID:Addgene_1876)
  • For your References section:

    Distinct roles for cyclin-dependent kinases in cell cycle control. van den Heuvel S, Harlow E. Science 1993 Dec 24;262(5142):2050-4. 10.1126/science.8266103 PubMed 8266103