Parkin
(Plasmid #187897)

• Purpose: Mammalian cell expression of untagged Parkin
• Depositing Lab: Erika Holzbaur
• Publication: Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7. doi: 10.1016/j.molcel.2023.08.005.

Backbone

• Vector backbone: pmCherry-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5300
• Modifications to backbone: mCherry fusion tag was deleted
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRKN
• Alt name: Park2
• Alt name: Parkin
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Mutation: None
• GenBank ID: NM_004562
• Entrez Gene: PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
• Promoter: CMV
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: Not available
• 3′ sequencing primer: Not available

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R. Youle, NIH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Subcloned from Addgene 23956.
Please visit https://www.biorxiv.org/content/10.1101/2022.06.21.496850v1 for bioRxiv preprint.