pBolux
(Plasmid
#188109)
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Purpose(Empty Backbone) Encodes a promoter-less Bacteroides-optimized lux cassette
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLYL01
- Backbone size (bp) 8036
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Modifications to backbonea Bacteroides optimized lux-cassette preceded by a SpeI restriction site was cloned into pLYL01 digested with BamHI and SalI.
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Vector typeBacterial Expression
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Selectable markersTetracycline in Bacteroides species
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberHigh Copy
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byIndividual components of the lux cassette were subcloned from "pmini-Tn5 luxCDABE Tc" originally published in Winson, M. K. et al., FEMS Microbiol Lett 163, 193-202 (1998).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBolux was a gift from Guy Townsend (Addgene plasmid # 188109 ; http://n2t.net/addgene:188109 ; RRID:Addgene_188109) -
For your References section:
Harnessing gut microbes for glycan detection and quantification. Modesto JL, Pearce VH, Townsend GE 2nd. Nat Commun. 2023 Jan 17;14(1):275. doi: 10.1038/s41467-022-35626-2. 10.1038/s41467-022-35626-2 PubMed 36650134
