Addgene - pET3a-Ub-K48C Plasmid Data

Plasmid 18897: pET3a-Ub-K48C

Gene/insert name:
Ubiquitin-K48C
Alt name:
UBC
Species:
H. sapiens (human)
Entrez Gene:
UBC (HMG20)
Mutation:
Lysine 48 is replaced with a Cysteine residue
Vector backbone:
pET3a
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4600
Cloning site 5':
NdeI
Site destroyed during cloning:
Unknown
Cloning site 3':
BamHI
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
BL21pJY2
High or low copy:
Low Copy
Person or lab that originally
cloned the gene/insert:
Beal R. has constructed this plasmid.
Sequence: View sequences (2)
Principal Investigator:
Cecile Pickart
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

Beal RE, Toscano-Cantaffa D, Young P, Rechsteiner M, Pickart CM. Biochemistry. 1998 Mar 3;37(9):2925-34

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Ubiquitin chain synthesis. Raasi et al (Methods Mol Biol. 2005 . 301():47-55. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18897" in your Materials and Methods section.