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pCaryP
(Plasmid #18945)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18945 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCary
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phiC31 attP
  • Alt name
    int
  • Alt name
    phiC31 phage attachment site
  • Species
    Streptomyces phage phiC31
  • Insert Size (bp)
    221

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    the plasmid pIJ8600 (a gift from Mervyn Bibb, John Innes Institute, Norwich, U.K.)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

attP residues 1123 to 1345
P-element residues 1386 to 1976
P-element residues 4560 to 4966

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCaryP was a gift from Michele Calos (Addgene plasmid # 18945 ; http://n2t.net/addgene:18945 ; RRID:Addgene_18945)
  • For your References section:

    Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth AC, Fish M, Nusse R, Calos MP. Genetics. 2004 Apr . 166(4):1775-82. 10.1534/genetics.166.4.1775 PubMed 15126397