pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro
(Plasmid
#189633)
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PurposeLentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 189633 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLentiCRISPRv2 backbone
- Backbone size w/o insert (bp) 9347
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameh7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
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gRNA/shRNA sequencenon targeting gRNA
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro was a gift from Manuel Kaulich (Addgene plasmid # 189633 ; http://n2t.net/addgene:189633 ; RRID:Addgene_189633) -
For your References section:
Optimized metrics for orthogonal combinatorial CRISPR screens. Cetin R, Wegner M, Luwisch L, Saud S, Achmedov T, Susser S, Vera-Guapi A, Muller K, Matthess Y, Quandt E, Schaubeck S, Beisel CL, Kaulich M. Sci Rep. 2023 May 6;13(1):7405. doi: 10.1038/s41598-023-34597-8. 10.1038/s41598-023-34597-8 PubMed 37149686
Map uploaded by the depositor.
