Addgene - p2xFlag CMV2 Lats1 Plasmid Data

Plasmid 18971: p2xFlag CMV2 Lats1

Gene/insert name:
Large tumor suppressor kinase 1
Alt name:
Lats1
Insert size:
3393
Species:
H. sapiens (human)
GenBank ID:
NM_004690
Entrez Gene:
LATS1 (WARTS, wts)
Fusion protein or tag:
2xFlag
Terminal:
N terminal on backbone
Mutation:
*see comments
Vector backbone:
p2xFlag CMV2
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
KpnI
Site destroyed during cloning:
Unknown
Cloning site 3':
XbaI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Marius Sudol
Terms and Licenses:
MTA

Comments:

*note that a user discovered a S1111L mutation in this insert. The origin of this substitution is unknown, as is its effect on function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mst2 and Lats kinases regulate apoptotic function of YAP. Oka et al (J Biol Chem. 2008 Jul 17. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18971" in your Materials and Methods section.