pcDNA4/HisMaxB-YAP2-2nd WW mutant
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18999||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5300
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameYes-kinase associated protein
Alt nameYAP2, YAP65
Alt nameYAP2 isoform
SpeciesH. sapiens (human)
Insert Size (bp)1467
MutationTryptophan 258 changed to Alanine, and Proline 261 changed to Alanine
Entrez GeneYAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA4/HisMaxB-YAP2-2nd WW mutant was a gift from Marius Sudol (Addgene plasmid # 18999 ; http://n2t.net/addgene:18999 ; RRID:Addgene_18999)
For your References section:Mst2 and Lats kinases regulate apoptotic function of YAP. Oka T, Mazack V, Sudol M. J Biol Chem. 2008 Jul 17. ():. 10.1074/jbc.M804380200 PubMed 18640976
Map uploaded by the depositor.