pAAV-MPRA-MluI-SpeI-EcoRI
(Plasmid
#190196)
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Purpose(Empty Backbone) Empty vector for inserting MPRA libraries into AAV
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV-hSyn-EGFP
- Backbone size (bp) 4061
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Modifications to backboneWe digested pAAV-hSyn-EGFP using MluI-HF and EcoR1-HF to remove eGFP from the original backbone, and ligated in an oligo that contains the sequences for MluI, SpeI, and EcoRI restriction sites.
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Vector typeMammalian Expression, AAV ; massively parallel reporter assay (MPRA)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsUse Endura Electrocompetent Cells (Lucigen, cat#60242-1). Grow 10 hours at 30C.
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Intended for MPRA libraries.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-MPRA-MluI-SpeI-EcoRI was a gift from Hyejung Won (Addgene plasmid # 190196 ; http://n2t.net/addgene:190196 ; RRID:Addgene_190196) -
For your References section:
Systematic investigation of allelic regulatory activity of schizophrenia-associated common variants. McAfee JC, Lee S, Lee J, Bell JL, Krupa O, Davis J, Insigne K, Bond ML, Zhao N, Boyle AP, Phanstiel DH, Love MI, Stein JL, Ruzicka WB, Davila-Velderrain J, Kosuri S, Won H. Cell Genom. 2023 Sep 15;3(10):100404. doi: 10.1016/j.xgen.2023.100404. eCollection 2023 Oct 11. 10.1016/j.xgen.2023.100404 PubMed 37868037
Map uploaded by the depositor.
