mADAM17 in pcDNA3.1
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19141||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesM. musculus (mouse)
Insert Size (bp)2480
Entrez GeneAdam17 (a.k.a. CD156b, Tace)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mADAM17 in pcDNA3.1 was a gift from Zena Werb (Addgene plasmid # 19141 ; http://n2t.net/addgene:19141 ; RRID:Addgene_19141)
For your References section:The low affinity IgE receptor (CD23) is cleaved by the metalloproteinase ADAM10. Lemieux GA, Blumenkron F, Yeung N, Zhou P, Williams J, Grammer AC, Petrovich R, Lipsky PE, Moss ML, Werb Z. J Biol Chem. 2007 May 18. 282(20):14836-44. 10.1074/jbc.M608414200 PubMed 17389606