pICSL1P7_crU626
(Plasmid #191782)

• Purpose: Cas12a guide expression in dicots
• Depositing Lab: Wendy Harwood
• Publication: Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi: 10.1007/978-1-0716-3131-7_16.

Backbone

• Vector backbone: n/a
• Backbone size w/o insert (bp): 4372
• Vector type: Plant Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
• Alt name: gRNA to be added by user
• Species: Lachnospiraceae bacterium
• Insert Size (bp): 1605

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pICSL1P7_crU626.gb
• A portion of this plasmid was derived from a plasmid made by: Made by Mark Youles

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

User inserts own guide. Level 1 position 7