|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19180||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4823
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFLAG tetracysteine tag
Insert Size (bp)78
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SacII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PL-452 C-Flag-4C was a gift from Patrik Verstreken (Addgene plasmid # 19180 ; http://n2t.net/addgene:19180 ; RRID:Addgene_19180)
For your References section:Recombineering-mediated tagging of Drosophila genomic constructs for in vivo localization and acute protein inactivation. Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P. Nucleic Acids Res. 2008 Aug 1. ():. 10.1093/nar/gkn486 PubMed 18676454