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HA-VHL L188V-pBabe-puro
(Plasmid #19241)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19241 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    HA-pBabe-puro
  • Backbone size w/o insert (bp) 5202
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    von Hippel-Lindau tumor suppressor (VHL)
  • Alt name
    vHL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    652
  • Mutation
    changed Leucin 188 to Valin
  • GenBank ID
    NM_000551
  • Entrez Gene
    VHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)
  • Tag / Fusion Protein
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer pBabe-5'
  • 3′ sequencing primer pBabe-3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-VHL L188V-pBabe-puro was a gift from William Kaelin (Addgene plasmid # 19241 ; http://n2t.net/addgene:19241 ; RRID:Addgene_19241)
  • For your References section:

    Hypoxia-inducible factor linked to differential kidney cancer risk seen with type 2A and type 2B VHL mutations. Li L, Zhang L, Zhang X, Yan Q, Minamishima YA, Olumi AF, Mao M, Bartz S, Kaelin WG. Mol Cell Biol. 2007 Aug . 27(15):5381-92. 10.1128/MCB.00282-07 PubMed 17526729