pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR
(Plasmid
#192636)
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PurposeExpresses ERT2-CreN59-nMag-NLS with mCherry marker.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192636 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSIN
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Vector typeMammalian Expression, Lentiviral, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
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SpeciesSynthetic
- Promoter EF1alpha
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ttcaggtgtcgtgaggaattg
- 3′ sequencing primer cgccctagatgcatgcggatc (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR was a gift from Yingxiao Wang (Addgene plasmid # 192636 ; http://n2t.net/addgene:192636 ; RRID:Addgene_192636) -
For your References section:
An AND-Gated Drug and Photoactivatable Cre-loxP System for Spatiotemporal Control in Cell-Based Therapeutics. Allen ME, Zhou W, Thangaraj J, Kyriakakis P, Wu Y, Huang Z, Ho P, Pan Y, Limsakul P, Xu X, Wang Y. ACS Synth Biol. 2019 Oct 18;8(10):2359-2371. doi: 10.1021/acssynbio.9b00175. Epub 2019 Oct 8. 10.1021/acssynbio.9b00175 PubMed 31592660
Map uploaded by the depositor.
