Addgene - pCMV-HSF1-flag Plasmid Data

Plasmid 1932: pCMV-HSF1-flag

Gene/insert name:
heat shock factor 1
Alt name:
HSF1
Insert size:
1600
Species:
H. sapiens (human)
Entrez Gene:
HSF1 (HSTF1)
Fusion protein or tag:
flag
Terminal:
C terminal on insert
Vector backbone:
pCMV5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3600
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
flag added to pCMV-huHSF-B from Carl Wu (NIH)
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Robert Kingston
Terms and Licenses:
MTA

Comments:

C-terminal epitope tagged hHSF1. SDYKDDDK*. Flag added by PCR. Total vector size is 5.2kb. (Kingston #1036)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1932" in your Materials and Methods section.