1
Plasmid 1932: pCMV-HSF1-flag
  • heat shock factor 1

  • HSF1

  • 1600

  • H. sapiens (human)

  • HSF1 (HSTF1)

  • flag

  • C terminal on insert

  • pCMV5
    (Search Vector Database)

  • Mammalian Expression

  • 3600

  • EcoRI

  • No

  • EcoRI

  • No

  • CMV-F List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • flag added to pCMV-huHSF-B from Carl Wu (NIH)

  • View sequences (1)
  • View map

  • Robert Kingston

  • UBMTA

Comments: 

C-terminal epitope tagged hHSF1. SDYKDDDK*. Flag added by PCR. Total vector size is 5.2kb. (Kingston #1036)

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Repression of human heat shock factor 1 activity at control temperature by phosphorylation. Knauf et al (Genes Dev 1996 Nov 1;10(21):2782-93. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1932" in your Materials and Methods section.

Discontinued

Addgene no longer distributes this plasmid. Contact help@addgene.org for more information