- Backbone size w/o insert (bp) 3600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
Gene/Insert nameheat shock factor 1
SpeciesH. sapiens (human)
Insert Size (bp)1600
Entrez GeneHSF1 (a.k.a. HSTF1)
/ Fusion Protein
- flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byflag added to pCMV-huHSF-B from Carl Wu (NIH)
Terms and Licenses
C-terminal epitope tagged hHSF1. SDYKDDDK*. Flag added by PCR. Total vector size is 5.2kb. (Kingston #1036)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-HSF1-flag was a gift from Robert Kingston (Addgene plasmid # 1932)
For your References section:Repression of human heat shock factor 1 activity at control temperature by phosphorylation. Knauf U, Newton EM, Kyriakis J, Kingston RE. Genes Dev 1996 Nov 1;10(21):2782-93. 10.1101/gad.10.21.2782 PubMed 8946918
Map uploaded by the depositor.