pCMV-HSF1-flag
(Plasmid #1932)

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    heat shock factor 1
  • Alt name
    HSF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Entrez Gene
    HSF1 (a.k.a. HSTF1)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    flag added to pCMV-huHSF-B from Carl Wu (NIH)
  • Terms and Licenses

Depositor Comments

C-terminal epitope tagged hHSF1. SDYKDDDK*. Flag added by PCR. Total vector size is 5.2kb. (Kingston #1036)

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-HSF1-flag was a gift from Robert Kingston (Addgene plasmid # 1932)
  • For your References section:

    Repression of human heat shock factor 1 activity at control temperature by phosphorylation. Knauf U, Newton EM, Kyriakis J, Kingston RE. Genes Dev 1996 Nov 1;10(21):2782-93. 10.1101/gad.10.21.2782 PubMed 8946918