EGFP-Scarlet-I
(Plasmid
#193554)
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PurposeExpresses mEGFP-mScarlet-I in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193554 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Total vector size (bp) 5332
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemScarlet-I
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Insert Size (bp)681
- Promoter CMV
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Tag
/ Fusion Protein
- mEGFP (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-pArev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAddgene #85068
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.03.21.485138 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-Scarlet-I was a gift from Justin Taraska (Addgene plasmid # 193554 ; http://n2t.net/addgene:193554 ; RRID:Addgene_193554) -
For your References section:
A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells. Obashi K, Sochacki KA, Strub MP, Taraska JW. Nat Commun. 2023 Feb 9;14(1):732. doi: 10.1038/s41467-023-36304-7. 10.1038/s41467-023-36304-7 PubMed 36759616
