pAG300
(Plasmid
#19363)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19363 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepVT102U
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Backbone manufacturerVernet et al 1987
- Backbone size (bp) 6400
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Modifications to backboneSfiIA (GGCCATTACGGCC) and SfiIB (GGCCGCCTCGGCC) sites added for unidirectional cloning.
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG300 was a gift from Holger Deising (Addgene plasmid # 19363 ; http://n2t.net/addgene:19363 ; RRID:Addgene_19363) -
For your References section:
Sfp-type 4'-phosphopantetheinyl transferase is indispensable for fungal pathogenicity. Horbach R, Graf A, Weihmann F, Antelo L, Mathea S, Liermann JC, Opatz T, Thines E, Aguirre J, Deising HB. Plant Cell. 2009 Oct;21(10):3379-96. doi: 10.1105/tpc.108.064188. Epub 2009 Oct 30. 10.1105/tpc.108.064188 PubMed 19880801