pGL4.0-TERT_-100_-79
(Plasmid
#193785)
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Purposeluciferase reporter for duplicated TERT promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193785 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGL4.0
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Backbone manufacturerPromega
- Total vector size (bp) 4418
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTERT promoter
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Alt nametelomerase reverse transcriptase
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SpeciesH. sapiens (human)
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Entrez GeneTERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
- Promoter TERT
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer Luc2-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4.0-TERT_-100_-79 was a gift from Joseph Costello (Addgene plasmid # 193785 ; http://n2t.net/addgene:193785 ; RRID:Addgene_193785) -
For your References section:
Conserved features of TERT promoter duplications reveal an activation mechanism that mimics hotspot mutations in cancer. Barger CJ, Suwala AK, Soczek KM, Wang AS, Kim MY, Hong C, Doudna JA, Chang SM, Phillips JJ, Solomon DA, Costello JF. Nat Commun. 2022 Sep 16;13(1):5430. doi: 10.1038/s41467-022-33099-x. 10.1038/s41467-022-33099-x PubMed 36114166
