p2T-cmv-SpCas9-TadCBEa-BlastR
(Plasmid
#193847)
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PurposeExpress TadCBEa in mammalian cells with BlastR selection, Tol2 integration
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193847 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep2T-CMV-BlastR
- Total vector size (bp) 10289
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTadCBEa
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Insert Size (bp)5380
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2T-cmv-SpCas9-TadCBEa-BlastR was a gift from David Liu (Addgene plasmid # 193847 ; http://n2t.net/addgene:193847 ; RRID:Addgene_193847) -
For your References section:
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity. Neugebauer ME, Hsu A, Arbab M, Krasnow NA, McElroy AN, Pandey S, Doman JL, Huang TP, Raguram A, Banskota S, Newby GA, Tolar J, Osborn MJ, Liu DR. Nat Biotechnol. 2022 Nov 10. pii: 10.1038/s41587-022-01533-6. doi: 10.1038/s41587-022-01533-6. 10.1038/s41587-022-01533-6 PubMed 36357719