pTREtight2-HA_GDP1
(Plasmid #19408)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pTREtight2
  • Backbone size w/o insert (bp) 2932
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a, 37°C
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    GDP1
  • Alt name
    KLLA0F09141g hypothetical protein
  • Species
    Kluyveromyces lactis
  • Insert Size (bp)
    1071
  • GenBank ID
    AJ430565
  • Entrez Gene
    KLLA0F09141g
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Term
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTREtight2-HA_GDP1 was a gift from Markus Ralser (Addgene plasmid # 19408)