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(Plasmid #19408)

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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone size w/o insert (bp) 2932
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    DH5a, 37°C
  • Copy number
    High Copy

Sequence Information


  • Gene/Insert name
  • Alt name
    KLLA0F09141g hypothetical protein
  • Species
    Kluyveromyces lactis
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Term
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTREtight2-HA_GDP1 was a gift from Markus Ralser (Addgene plasmid # 19408)